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Immunology and Infection

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JoVE Journal Immunology and Infection
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
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Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Article DOI: 10.3791/52556-v • 11:21 min • March 12th, 2015
March 12th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Microbiology and Immunology, New York Medical College
* These authors contributed equally

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Summary
Automatic Translation

Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

Tags

Genetics Screens Macrophages Toxoplasma Gondii IFN-dependent Cell Autonomous Immunity Parasite Invasion Parasitophorous Vacuole Innate Immune Cells Forward Genetic Screen Fitness Defect Macrophage Activation Parasite Mutants Resistance To Macrophage Activation Parasite Phenotypes
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