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JoVE Journal Medicine
Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
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Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Article DOI: 10.3791/52656-v • 11:42 min • March 15th, 2015
March 15th, 2015 •
1, 1, 1, 2, 1, 1
1Department of Pediatrics, Stanford University School of Medicine, 2Department of Orthopaedic Surgery, Stanford University School of Medicine

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Summary
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Protocols are described for studying breast cancer cell migration, proliferation and colonization in a human bone tissue explant model system.

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Culturing Human Femur Tissue Breast Cancer Cell Colonization Metastatic Niche Microenvironment Bone Tissue Fragments Total Hip Replacement Surgeries Luciferase Enhanced Green Fluorescent Protein (EGFP) Migration Proliferation Patterns Bioluminescence Imaging (BLI) Fluorescence Microscopy Flow Cytometry Architecturally Intact Tissue Microenvironment Human Cell Types Monitoring Perturbation Short-term Culture Model System
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