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Immunology and Infection

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JoVE Journal Immunology and Infection
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
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High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

Article DOI: 10.3791/52779-v • 11:03 min • June 18th, 2016
June 18th, 2016 •
*1, *1, *1, 1, 1
1Department of Infectious Diseases, University of Georgia
* These authors contributed equally

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Summary
Automatic Translation

High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.

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High Throughput Measurement Extracellular DNA Release Quantitative NET Formation Human Neutrophils In Vitro Inflammation Inflammatory Diseases Mechanism And Regulation Neutrophil Extracellular Trap Formation Signaling Pathways NET Inhibitory Molecules NET Stimulating Molecules Isolate Neutrophils Blood Dextran Leukocyte Rich Upper Phase Clean Conical Tubes Sterile PBS Centrifuge Pellet Resuspension Percoll Gradients
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