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JoVE Journal Medicine
Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo
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Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Article DOI: 10.3791/52794-v • 08:07 min • June 23rd, 2015
June 23rd, 2015 •
1, 1, 2, 3, 1
1Department of Cellular and Structural Biology, The University of Texas Health Science Center San Antonio, 2School of Medicine, The University of Texas Health Science Center San Antonio, 3Cell & Tissue Imaging Center, St. Jude Children’s Research Hospital

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Summary
Automatic Translation

Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.

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Rose Bengal Photothrombosis Confocal Optical Imaging In Vivo Imaging Techniques Physiological Assessment CNS Cellular Responses Stroke Induction Ischemic Event Laser Excitation Photosensitive Dye Thin-skulled Cranial Window Somatosensory Cortex Surgical Preparation Confocal Microscope Longitudinal In Vivo Studies
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