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Journal / Developmental Biology / Direct Induction of Hemogenic Endothelium and…
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JoVE Journal Developmental Biology
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Article DOI: 10.3791/52910-v • 08:14 min • December 3rd, 2015
December 3rd, 2015 •
1, 1, 2
1Primate Research Center, University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health

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Summary
Automatic Translation

This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

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Direct Induction Hemogenic Endothelium Blood Transcription Factors Human Pluripotent Stem Cells Endothelial-hematopoietic Transition Hematopoietic Specification ETV2 GATA1 GATA2 TAL1 LMO2 Pan-myeloid Potential Erythroid And Megakaryocytic Potential Differentiation Culture Dish
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