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Isolation and Primary Culture of Mouse Aortic Endothelial Cells
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Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Article DOI: 10.3791/52965-v • 08:20 min • December 19th, 2016
December 19th, 2016 •
1, 2, 1
1Center for Molecular Medicine and Genetics, Wayne State University, 2Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine, Central South University

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Summary
Automatic Translation

The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

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