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Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
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Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Article DOI: 10.3791/53059-v • 10:00 min • October 12th, 2015
October 12th, 2015 •
1, 1, 1, 1, 1, 1
1Department of Physiology, Medical College of Wisconsin

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Enzymatic microelectrode biosensors enable real-time measurements of extracellular cell signaling in biologically-relevant concentrations. The following protocols extend the applications of biosensors to the ex vivo and in vivo detection of ATP and H2O2 in the kidney.

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Enzymatic Biosensors ATP Measurement H2O2 Measurement Kidney Signaling Microelectrode Biosensors Brain Slices Neuronal Cell Cultures Whole Organs Sensor Design Blood-perfused Kidneys ATP And H2O2 Signaling Rat Kidney Interstitium Ex Vivo Protocol In Vivo Protocol Enzymatic Biolayer Permselectivity Layer Glycerol Kinase Glycerol-3-phosphate Oxidase Platinum/iridium Wire Electrode Mediator Coated Gold Electrode Real-time Amperometry
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