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Using Caco-2 Cells to Study Lipid Transport by the Intestine

Article DOI: 10.3791/53086-v • 07:00 min • August 20th, 2015
August 20th, 2015 •
1, 2
1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, 2Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University

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Summary
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Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.

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Caco-2 Cells Lipid Transport Intestine Dietary Fat Absorption In Vitro Model Animal Model Enterocytes Colon Carcinoma Cells Permeable Membrane System Apical Compartment Basolateral Compartment Lipoproteins Lentivirus Expression System Gene Expression
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