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Immunology and Infection

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JoVE Journal Immunology and Infection
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Article DOI: 10.3791/53364-v • 11:50 min • October 1st, 2015
October 1st, 2015 •
1, 1, 1, 1, 1, 1
1Department of Biology, University of Alabama at Birmingham

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Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.

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Bacterial Leaf Infiltration Assay Plant Defense Responses Arabidopsis Thaliana Pseudomonas Syringae Pathosystem Localized Programmed Cell Death Systemic Acquired Resistance Pathogen Attack Arabidopsis Gene Quantitatively Assess Pathogen Growth Infected Tissue Hemibiotrophic Bacterium Pseudomonas Syringae Pv. Maculicola ES4326 Model Pathogen Molecular Mechanisms Inoculation Methods Syringe Infiltration Dip Inoculation Spray Vacuum Infiltration Flood Inoculation Enhanced Disease Susceptibility (EDS) Adult Soil-grown Arabidopsis Plants Disease Susceptibility Screening Salicylic Acid (SA)-Triggered Immunity (STI) MAMP-Triggered Immunity (MTI)
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