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JoVE Journal Neuroscience
Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Article DOI: 10.3791/53369-v • 10:31 min • September 29th, 2017
September 29th, 2017 •
1, 2, 2, 3
1University of Michigan Medical School, 2Department of Neurology, University of Michigan, 3Department of Internal Medicine, University of Michigan

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Summary
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This protocol uses three-dimensional (3D) imaging and analysis techniques to visualize and quantify nerve-specific mitochondria. The techniques are applicable to other situations where one fluorescent signal is used to isolate a subset of data from another fluorescent signal.

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Three-dimensional Imaging Analysis Mitochondria Human Intraepidermal Nerve Fibers Visualize Quantify Neurology Neurological Complications Fluorescence Immunohistochemistry Staining Skin Biopsies Stock Signal Enhancer Solution Nonspecific Binding Secondary Antibodies Phosphate Buffered Saline 5%BSA Blocking Solution Primary Antibodies PGP9.5 And PDH Rinsing Solution
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