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Efficiënte zoogdiercelexpressie en Single-step Zuivering van extracellulaire Glycoproteïnen voor kristallisatie

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Efficiënte zoogdiercelexpressie en Single-step Zuivering van extracellulaire Glycoproteïnen voor kristallisatie

Article DOI: 10.3791/53445-v • 07:08 min • December 23rd, 2015

December 23rd, 2015 •

Daniel L. Kober*^1,2,3, Zeynep Yurtsever*^2,3,4,5, Thomas J. Brett^2,3,5,6,7

¹Molecular Microbiology and Microbial Pathogenesis Program, Washington University School of Medicine, ²Department of Internal Medicine, Washington University School of Medicine, ³Drug Discovery Program in Pulmonary and Critical Care Medicine, Washington University School of Medicine, ⁴Biochemistry Program, Washington University School of Medicine, ⁵Center for the Investigation of Membrane Excitability Diseases, Washington University School of Medicine, ⁶Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, ⁷Department of Cell Biology and Physiology, Washington University School of Medicine

* These authors contributed equally

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Biochemie zoogdiereiwit expressie nikkel affiniteitschromatografie glycosylering macromoleculaire kristallografie

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