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Developmental Biology

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JoVE Journal Developmental Biology
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Article DOI: 10.3791/53624-v • 11:53 min • March 10th, 2016
March 10th, 2016 •
1, 1, 1, 1
1Department of Animal Science and Technology, National Taiwan University

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Summary
Automatic Translation

To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.

Tags

Avian Endodermal Epithelial Cells Yolk Sac Membrane Japanese Quail Embryos Ex Vivo Explant Procedure Nutrient Utilization Lipid Utilization Yolk Lipids Avian Embryogenesis Functional Endodermal Epithelial Cells Avian Embryonic Development 10x PBS Solution Growth Medium EECs DMEM/F-12 Newborn Calf Serum Penicillin Streptomycin Amphotericin Solution PH Meter HCl Adjustment Fertilized Quail Eggs
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