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JoVE Journal Neuroscience
Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
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Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Article DOI: 10.3791/53866-v • 12:03 min • June 7th, 2016
June 7th, 2016 •
1, 2, 3, 2
1Farquhar College of Arts and Sciences, Nova Southeastern University, 2Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, 3School of Medicine, University of Colorado

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Summary
Automatic Translation

Here we present a protocol to describe the localization of angiotensin II Type 1 receptors in the rat brain by quantitative, densitometric, in vitro receptor autoradiography using an iodine-125 labeled analog of angiotensin II.

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Receptor Autoradiography Angiotensin II Receptors Brain Sections Localization Quantification Histological Identification Brain Structures Behavior Cognitive Function Cardiovascular System Novel Therapies Neurodegenerative Diseases Cardiovascular Diseases Advantages Quantitative Qualitative Specificity Hormone Receptor Systems Drugs Of Abuse Brain Molds Storage Temperature Cryostat
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