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JoVE Journal Developmental Biology
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Article DOI: 10.3791/53974-v • 06:02 min • June 10th, 2016
June 10th, 2016 •
1,2, 2, 2, 2, 2, 2
1Department of Clinical Laboratory, The University of Tokyo Hospital, 2Department of Respiratory Medicine, The University of Tokyo Hospital

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Summary
Automatic Translation

Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.

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In Vitro Assay Contractile Function Mesenchymal Cells Epithelial-mesenchymal Transition Inflammatory Cytokine Idiopathic Pulmonary Fibrosis Airway Remodeling Technique High Content Hideyuki Takeshima Kosuke Makita A-549 Human Lung Epithelial Cells PBS Trypsin-EDTA Cell Culture Medium Centrifuge EMT Induction TGF Beta 1 TNF Alpha
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