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JoVE Journal Neuroscience
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
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Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Article DOI: 10.3791/54025-v • 10:28 min • June 30th, 2016
June 30th, 2016 •
1, 1, 2, 1
1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 2W.M. Keck Science Department, Claremont McKenna, Pitzer & Scripps Colleges

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Summary
Automatic Translation

Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive results.

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Optical Clearing CLARITY Mouse Central Nervous System Neuroscience 3D Morphology Connectivity Microscopy Cost Effective Consistent Results Imaging Chamber Construction Paraformaldehyde Fixed Brain And Spinal Cord Tissue Fluorescent Proteins Hydrogel Solution Incubation Period Deoxygenate Sample
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