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Journal / Developmental Biology / Protocol for the Direct Conversion of Murine…
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Developmental Biology

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JoVE Journal Developmental Biology
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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Article DOI: 10.3791/54277-v • 08:57 min • July 25th, 2016
July 25th, 2016 •
1, 1
1Department of Developmental Pathology, Institute of Pathology, University of Bonn Medical School

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Summary
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Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

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Murine Embryonic Fibroblasts Trophoblast Stem Cells Induced Trophoblast Stem Cells Transgenic Expression Poly-L-lysine Solution 293 T Cells Transfection Medium Chloroquine Solution Lentiviral Vector HEPES Buffer
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