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Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation
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Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation

Article DOI: 10.3791/54322-v • 09:23 min • August 18th, 2016
August 18th, 2016 •
1, 1,2, 1,3, 1,2
1CHU de Québec Research Centre, 2Department of Molecular Medicine, Laval University, 3Department of Surgery, Laval University

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Summary
Automatic Translation

Cancer cells are embedded in a collagen gel and then sandwiched in an acellular fibrin gel to generate a 3D culture system in which the invasiveness and formation of satellite tumors may be monitored.

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Three-dimensional Culture Assay Cancer Cell Invasiveness Satellite Tumor Formation Collagen Embedded Cancer Cells Fibrin Gel 3D Culture System Monitoring Satellite Tumor Invasiveness Monitoring Satellite Tumor Formation Anti-cancer Cell Loss Extra Cellular Matrix 3D Cell Culture Model Freeze Dried Rat Tail Tendon Type One Collagen Tumor Cells Enzyme Detaching Solution Trypan Blue Exclusion Cell Concentration DMEM Single Cell Suspension 96 Well Plate
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