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Immunology and Infection

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JoVE Journal Immunology and Infection
Reliable and High Efficiency Extraction of Kidney Immune Cells
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Journal (Immunology and Infection)
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Reliable and High Efficiency Extraction of Kidney Immune Cells

Article DOI: 10.3791/54368-v • 09:15 min • August 19th, 2016
August 19th, 2016 •
1, 2, 3, 3
1Division of Nephrology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 2Division of Biomedical Sciences, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 3Division of Endocrinology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital

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Summary
Automatic Translation

Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.

Tags

Kidney Immune Cells Cell Isolation Flow Cytometry Collagenase Digestion Experimental Analysis Kidney Immunobiology Cytokine Secretion Chemokine Secretion Kidney Disease Immune Cell Isolation Multicolor Flow Cytometry Kidney Immunobiology Field Liver Cells Brain Cells Research Specialist Anesthetized Mouse Abdominal Cavity
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