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Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Article DOI: 10.3791/54756-v • 12:06 min • February 1st, 2017
February 1st, 2017 •
1, 1, 1
1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

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Summary
Automatic Translation

A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.

Tags

Molecular Diffusion Plasma Membranes Primary Lymphocytes Fluorescence Correlation Spectroscopy Labeled Protein Immunology Cell Biology Natural Killer Cells Membrane Receptor Dynamics Live Cells Immunotherapy Of Cancer Molecular Movement Immune Cells Primary Cells Cell Lines Confocal Laser Scanning Microscope Water Objective Lens Software Expert Mode Scan New Images Configuration Control Scan Control ConfoCor Tab FCS Measurements Halogen Lamp Lasers
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