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Journal / Developmental Biology / Analisi dei vasi coronarici in sgomberati cuori…
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Developmental Biology

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JoVE Journal Developmental Biology
Analisi dei vasi coronarici in sgomberati cuori embrionali
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Analisi dei vasi coronarici in sgomberati cuori embrionali

Article DOI: 10.3791/54800-v • 08:25 min • December 7th, 2016
December 7th, 2016 •
1, 1, 2, 1
1Developmental Biology of Birth Defects, UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building, University of Edinburgh

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Vi presentiamo un protocollo per l'analisi dei vasi coronarici, in tutto cuori murini embrionali fino a E15.5, usando metodi di colorazione immunologici standard, seguiti da gioco ottica e la microscopia confocale. Questa tecnica permette la visualizzazione dei vasi sanguigni durante tutto cuore senza la necessità di analizzare tempo di sezioni seriali.

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Biologia dello Sviluppo Numero 118 vasi coronarici BABB PECAM1 cuore embrionale immunofluorescenza wholemount compensazione ottica microscopia confocale
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