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Immunology and Infection

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JoVE Journal Immunology and Infection
Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
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Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research

Article DOI: 10.3791/55206-v • 09:42 min • April 19th, 2017
April 19th, 2017 •
1, 1, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine

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Summary
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This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.

Tags

DNA Delivery System Acufection Skin Research Immune Related Genes Cutaneous Inflammation Novel Gene Function Cost Effective Plasmid DNA Antibiotic Supplemented LB Medium Overnight Incubation Endotoxin Free Plasmid Preparation Kit Sterile PBS Anesthetizing The Mouse Veterinary Ophthalmologic Ointment Depilation Keratin Proteins Cotton Swab
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