JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.