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JoVE Journal Developmental Biology
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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Article DOI: 10.3791/55297-v • 10:18 min • June 26th, 2017
June 26th, 2017 •
1,2,3, 4, 4, *1, *4
1Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, 2Department of Neuroscience; Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine, 3Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, 4Department of BioSciences, Università degli Studi di Milano
* These authors contributed equally

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Summary
Automatic Translation

Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.

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Biosensing Motor Neuron Membrane Potential Live Zebrafish Embryos FRET-based GEVI Excitable Cells Noninvasive Physiological Conditions Neurological Disorders Genetically-encoded Voltage Indicators Spinal Neuron Excitability In-vitro Systems In-vivo Systems Imaging Studies PCR Mixture Mermaid Biosensor Coding Sequence Gel Purification Kit DNA Fragment PCMV-SC Blunt Vector SmaI Restriction Enzyme
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