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Journal / Genetics / Generazione di fusioni proteina fluorescente in
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Genetics

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JoVE Journal Genetics
Generazione di fusioni proteina fluorescente in
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Generazione di fusioni proteina fluorescente in

Article DOI: 10.3791/55333-v • 09:27 min • March 4th, 2017
March 4th, 2017 •
1, 2, 1
1Department of Pediatrics, University of Minnesota, 2Department of Molecular Microbiology and Biotechnology, Tel Aviv University

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Modificazione genetica PCR-mediata può essere utilizzato per generare fusioni di proteine fluorescenti a Candida specie, che facilita la visualizzazione e la quantificazione delle cellule di lievito e proteine. Qui, vi presentiamo una strategia per la costruzione di una fusione proteina fluorescente (Eno1-FP) in Candida parapsilosis.

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Genetica Numero 121, PCR-mediata modificazione genetica la proteina fluorescente, Litio acetato di trasformazione,
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