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JoVE Journal Genetics
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Article DOI: 10.3791/55369-v • 07:41 min • October 2nd, 2017
October 2nd, 2017 •
1, 1, 1
1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University

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Summary
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Sequential salt extraction of chromatin bound proteins is a useful tool for determining the binding properties of large protein complexes. This method can be employed to evaluate the role of individual subunits or domains in the overall affinity of a protein complex to bulk chromatin.

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Sequential Salt Extraction Protein Binding Profiles Chromatin Modifying Complexes Bulk Chromatin Genetic Manipulation Chemical Manipulation Environmental Manipulation Epigenetic Regulators Chromatin Immunoprecipitation (ChIP) Hypotonic Buffer Protease Inhibitors Microcentrifuge Tubes Centrifuge Supernatant Salt-free Mo
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