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JoVE Journal Neuroscience
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Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Article DOI: 10.3791/55488-v • 08:15 min • May 22nd, 2017
May 22nd, 2017 •
1,2,3, 1, 1, 1,2,3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2PhD Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 3Life Science Center of Tsukuba Advanced Research Alliance (TARA), University of Tsukuba

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The investigation of membrane trafficking is crucial for understanding neuronal functions. Here, we introduce a method for quantifying vesicle motility in neurons. This is a convenient method that can be adapted to the quantification of membrane trafficking in the nervous system.

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Quantification Endosome Lysosome Motility Cultured Neurons Fluorescent Probes Imaging Analysis Vesicle Motility Membrane Trafficking Petri Dish HBSS Uterus Fetuses Surgical Scissors Stereo Microscope Brains Meninges Cortices Cerebral Cortical Enzyme Solution Water Bath
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