JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.