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JoVE Journal Neuroscience
Exposure of the Pig CNS for Histological Analysis
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Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Article DOI: 10.3791/55511-v • 08:39 min • April 13th, 2017
April 13th, 2017 •
1, 2, 2, 2, 2, 2
1Department of Neurosurgery, Clinical Institute of Medicine, Aalborg University Hospital, 2Center of Experimental Neuroscience (Cense), Department of Neurosurgery, Institute of Clinical Medicine, Aarhus University Hospital

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Summary
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The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis.

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Exposure Pig CNS Histological Analysis Decapitation Skull Opening Brain Removal Intact Pig Brain Intact Pituitary Skull And Dura Opening Animal Anesthesia Euthanasia Midazolam Ketamine Sodium Pentobarbital Transcardial Perfusion Postmortem Procedures Welfare Precautions Scalpel Handle Number Four Scalpel Blades Number 23 Scalpel Handle Number Seven Scalpel Blades Number 11 Surgical Forceps KAIRison Bone Punch Bone Rongeur
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