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Personalized Needles for Microinjections in the Rodent Brain

Article DOI: 10.3791/55751-v • 10:06 min • January 24th, 2018
January 24th, 2018 •
*1, *1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara, 2Laboratory for the Technologies for Advanced Therapies (LTTA), University of Ferrara
* These authors contributed equally

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Summary
Automatic Translation

We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.

Tags

Personalized Needles Microinjections Rodent Brain Protocol Refined Methodology Reusable Quartz Needle Tissue Damage Reliable Delivery Deep Regions Neuroscience Neurodegenerative Diseases Psychiatric Diseases Gene Therapy Cell Therapy Viral Vectors Cost-effective Cleaning And Sterilization Process Laser Puller Microscope Table
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