Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Expansion and Adipogenesis Induction of Adipocyte …
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
Journal (Developmental Biology)
Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Journal (Developmental Biology)
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
 

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Article DOI: 10.3791/55818-v • 08:28 min • June 30th, 2017
June 30th, 2017 •
1, 2, 2, 1
1Department of Large Animal Clinical Sciences, Michigan State University, 2Department of Pharmacology and Toxicology, Michigan State University

Chapters

Summary
Automatic Translation

Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

Tags

Adipocyte Progenitors Perivascular Adipose Tissue Magnetic Activated Cell Sorting Differentiation Protocols Mature Adipocytes Isolation Technique Antigen-presenting Cells Fluorescence-activated Cell Sorting Rat Surgery Abdominal Cavity Mesenteric Resistance Vessels Thoracic Aorta Fat Pads Krebs-Ringer Bicarbonate Buffer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter