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JoVE Journal Bioengineering
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Article DOI: 10.3791/55957-v • 08:22 min • August 11th, 2017
August 11th, 2017 •
*1, *1,2, 1,3
1Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Department of Plastic and Reconstructive Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 3Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University
* These authors contributed equally

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Summary
Automatic Translation

We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.

Tags

Microfluidic Bioprinting Engineering Vascularized Tissues Organoids Biofabrication Three-dimensional Shape Secondary Cell Seeding Process Vascular Vat Cardiac Tissues Liver Skin Cancers Bioprinter Setup Dual-layer Concentric Microfluidic Print Head Needle Sheath Core Needle Outer Shell 23 Gauge Needle Metal Connector Epoxy Glue Extruder Polymethyl Methacrylate (PMMA) Holder
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