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Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Article DOI: 10.3791/55959-v • 09:15 min • November 21st, 2017
November 21st, 2017 •
1, 2, 1
1Department of Chemistry, McGill University, 2Department of Chemistry, York University

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Summary
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We present a protocol for rapid characterization of biomolecular folding and binding interactions with thermolabile ligands using differential scanning calorimetry.

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Biomolecular DSC Profiles Thermolabile Ligands Folding Interactions Binding Interactions Thermodynamic Parameters Differential Scanning Calorimetry Biocalorimetry Drug Design Ligand Titration Global Fitting Analysis Buffers Dialysis High-quality DSC Data
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