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Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Article DOI: 10.3791/56086-v • 06:26 min • December 7th, 2017
December 7th, 2017 •
*1,2,3, *1,4, 1,4, 1,5
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 2Department of Genetics and Complex Diseases, T. H. Chan School of Public Health, Harvard Medical School, 3Department of Cell Biology, Harvard Medical School, 4Sorbonne Universités, UPMC University Paris 06, 5Center for Studies in Physics and Biology, The Rockefeller University
* These authors contributed equally

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Summary
Automatic Translation

Many proteins in the cell sense and induce membrane curvature. We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro.

Tags

Nanotube Pulling Giant Unilamellar Vesicles Protein Interactions Curved Membranes Endocytosis Intracellular Trafficking Lipid Membranes Mechanical Properties Protein Membrane Interactions Experimental Chamber Glass Cover Slips Beta-casein Solution Passivation Light Microscope Aspiration Micropipette
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