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JoVE Journal Cancer Research
Evaluation of the Cell Invasion and Migration Process
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Journal (Cancer Research)
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
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Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

Article DOI: 10.3791/56337-v • 11:20 min • November 17th, 2017
November 17th, 2017 •
*1,2, *1,2, 1,2, 1, 1,3, 1,3, 1,3, 1,2, 1,3, 1,2
1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud
* These authors contributed equally

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Summary
Automatic Translation

This study reports two different methods for the analysis of cell invasion and migration: the Boyden chamber assay and the in vitro video microscope-based wound-healing assay. The protocols for these two experiments are described, and their benefits and disadvantages are compared.

Tags

Cell Invasion Cell Migration Scratch Wound Assay Boyden Chamber Assay Video Microscope In Vitro Assay Cell Confluence Culture Medium Cell Density Fetal Bovine Serum Medium
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