JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.