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JoVE Journal Developmental Biology
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Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

Article DOI: 10.3791/56345-v • 12:06 min • January 14th, 2018
January 14th, 2018 •
1, 1, 1
1Department of Pathobiological Sciences, University of Wisconsin, School of Veterinary Medicine

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This article describes a protocol for the large-scale axenic isolation and collection of free-swimming miracidia of the human blood fluke Schistosoma mansoni and their subsequent processing for introduction into in vitro culture.

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Mass Isolation In Vitro Cultivation Intramolluscan Stages Human Blood Fluke Schistosoma Mansoni Hatching Miracidia In Vitro Culture Schistosomiasis Snails Intermediate Hosts Mammals Definitive Hosts Larval Schistosomes Adult Schistosomes Parasitic Disease Mouse Model S.mansoni Eggs Snail-infected Miracidial Stage Step By Step Procedure Mass Isolation Of Miracidia In Vitro Cultivation Of Miracidia Infected Livers Tissue Blending
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