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JoVE Journal Cancer Research
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
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Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Article DOI: 10.3791/56418-v • 09:09 min • November 10th, 2017
November 10th, 2017 •
*1, *1, 2, 1
1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the use of centrifugal elutriation to separate primary acute lymphoblastic leukemia cells into different cell cycle phases.

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Centrifugal Elutriation Primary Acute Lymphoblastic Leukemia Cells Cell Cycle Phases Isolation Of Cells Cancer Therapeutics Anti-cancer Compounds Physical Properties Chemical Perturbations Strobe Assembly Centrifuge Flash Lamp Viewing Window Phillips Head Screws Thumb Screws Power Cord Rotors Centrifuge Drive Hub T Handle Hex Wrench Elutriation Chamber Counterbalance Rotating Seal Assembly Mounting Plate
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