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JoVE Journal Neuroscience
Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction
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Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

Article DOI: 10.3791/56493-v • 07:01 min • September 25th, 2017
September 25th, 2017 •
1, 1,2
1Department of Neurology, School of Medicine, Wayne State University, 2Department of Anatomy and Cell Biology, School of Medicine, Wayne State University

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Summary
Automatic Translation

Synaptic currents can be recorded focally from visualized synaptic boutons at the Drosophila third instar larvae neuromuscular junction. This technique enables monitoring the activity of a single synaptic bouton.

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Focal Macropatch Recordings Synaptic Currents Drosophila Larval Neuromuscular Junction Genetic Manipulations Synaptic Proteins Synaptic Transmission Limited Number Of Release Sites Fast Kinetics Microelectrode Puller Microscope Capillaries Inner Diameter Dust Accumulation Fire Polished Capillaries Micro Forge Bending Manipulator Filament Heat Value L-shaped Needle Torch Flame
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