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JoVE Journal Biochemistry
DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
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DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Article DOI: 10.3791/56528-v • 08:12 min • January 28th, 2018
January 28th, 2018 •
1, 1, 1
1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile

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Summary
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This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.

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DNA Staining Method Formazan Precipitation Blue Light Exposure SYBR Green I Nitro Blue Tetrazolium (NBT) Gel Stirring Polyacrylamide Gel Agarose Gel Blue Light Source Precipitate Bands DNA Concentration Quantification Office Scanner Image Analysis Software Calibration Curves Plasmid Loading
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