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JoVE Journal Developmental Biology
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
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Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Article DOI: 10.3791/56802-v • 12:46 min • December 6th, 2017
December 6th, 2017 •
1, 1
1Department of Biological Sciences, Dartmouth College

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This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.

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Fluorescence In Situ Hybridization (FISH) Arm Cohesion Prometaphase Metaphase I Drosophila Oocytes Meiotic Arm Cohesion Premature Loss Of Arm Cohesion Maternal Age Effect Humans Oocyte Aging Arm Probe Dissection Fixation Late-stage Oocytes PBSBTX Dissecting Dish Fixed Ovaries BSA-coated Tip Mature Oocytes Less Mature Oocytes Microfuge Tube Pasteur Pipette
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