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Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

Article DOI: 10.3791/56902-v • 07:57 min • March 15th, 2018
March 15th, 2018 •
1, 1, 1
1Graduate School of Life Sciences, Toyo University

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Summary
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Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS.

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Endogenous Auxin Cytokinin Internode Culture Ipecac Phytohormone Adventitious Shoot Formation Plant Tissue Culture Indole-3-acetic Acid Tissue Regeneration B5 Medium Gellan Gum Autoclave Surgical Scalpel Sterile Acrylic Plate Ipecac Plantlets 24 Degrees Celsius Light Intensity Dark Cycle Five Weeks Regions I-IV Microscope Adventitious Shoots Zirconia Bead
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