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JoVE Journal Immunology and Infection
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
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Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Article DOI: 10.3791/57013-v • 11:14 min • October 12th, 2018
October 12th, 2018 •
1,2, 2, 2, 1, 2
1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital, Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health

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Summary
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Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.

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Ex Vivo Infection Human Lymphoid Tissue Female Genital Mucosa Human Immunodeficiency Virus 1 Histoculture HIV Field Virus Transmission Pathogenesis Tissue Culture Disease Progression Spacial And Functional Relationships Gelatin Sponges Culture Plate Incubator
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