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Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres

Article DOI: 10.3791/57016-v • 09:31 min • January 29th, 2018
January 29th, 2018 •
1, 1,2, 3,4, 1,2, 1,2
1Department of Cell and Developmental Biology, University of Michigan Medical School, 2Department of Neurosurgery, University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program, University of Michigan Medical School, 4Department of Biology, University of Puerto Rico-Río Piedras Campus

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Summary
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Epigenetic mechanisms are frequently altered in glioma. Chromatin immunoprecipitation could be used to study the consequences of genetic alterations in glioma that result from changes in histone modifications which regulate chromatin structure and gene transcription. This protocol describes native chromatin immunoprecipitation on murine brain tumor neurospheres.

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Native Chromatin Immunoprecipitation Murine Brain Tumor Neurospheres Histone Marks Epigenetics Field Epigenetic Regulators Histone Modifications Antibody Specificity Dissecting Microscope Scalpel Forceps Petri Dish Cell Detachment Medium Cell Strainer Centrifuge Tube
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