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Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
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Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

Article DOI: 10.3791/57235-v • 08:15 min • June 20th, 2018
June 20th, 2018 •
1, 1, 1
1Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University

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Summary
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A method is shown here for the preparation of the tongue extracellular matrix (TEM) with efficient decellularization. The TEM can be used as functional scaffolds for the reconstruction of a tongue squamous cell carcinoma (TSCC) model under static or stirred culture conditions.

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Tongue Extracellular Matrix Tongue Squamous Cell Carcinoma Oral Cancer Research T-assist Model 2-D Cultured TSCC Cells Tumor Characteristics Clinical TSCC Histopathology Extracellular Matrix Cancer Survival Evasion Differentiation Visual Demonstration Mini Bioreactor Tissue Isolation Cell Lysis Freeze/thaw Method Surgical Needle Surgical Suture
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