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JoVE Journal Developmental Biology
Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo
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Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Article DOI: 10.3791/57290-v • 09:38 min • February 20th, 2018
February 20th, 2018 •
*1,2,3,4, *1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104, Centre National de la Recherche Scientifique, 3U964, Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development.

Tags

Endocardial Tissue Movements Cell Photoconversion Zebrafish Embryo Atrioventricular Canal Heart Valve Formation Cardiac Development Endocardial Cells Mutants Time-lapse Microscopy Procedure Protocol Mold Agarose Mounting Dish Mounting Medium Low Melting Point Agarose
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