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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Article DOI: 10.3791/57386-v • 09:00 min • May 2nd, 2018
May 2nd, 2018 •
1,2, 3, 4
1Department of Animal Health, Universidad Complutense de Madrid, 2Visavet Health Surveillance Centre, Universidad Complutense de Madrid, 3Department of Zoology, University of Oxford, 4Department of Microbiology, Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

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Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

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Multicopy Plasmids Evolution Antibiotic Resistance Experimental Method Microbiology Bacterial Innovations Molecular Biology Methods Simple Experimental Design E Coli MG1655 Strain Antibody Resistance Gene Lambda Phage ATTB Site PCR Amplification Isothermal Assembly Electrocompetent Cells Electroporation LB Broth Arabinose Microfuge Tube Culture LB Agar Plate
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