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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Article DOI: 10.3791/57740-v • 10:07 min • July 15th, 2018
July 15th, 2018 •
*1, *1, 2, 1, 1
1Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, 2Endocrinology and Diabetes Research Group, Biocruces Health Research Institute, UPV-EHU, CIBERDEM
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal biopsies.

Tags

Subcellular Fractionation Frozen Gastrointestinal Specimens Fresh Gastrointestinal Specimens Protein Localization Cellular Compartment Reproducible Fractionation Intestinal Biopsy Samples Protein Quantification Immuno Precipitation Studies Clinical Intestinal Samples Tissue-specific Proteins Disease-specific Proteins Nuclear Compartment Cytoplasmic Compartment Frozen Tissue Procedure Cold Homogenization Buffer Fresh Samples
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