JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We describe an optical assay for synaptic vesicle (SV) recycling in cultured neurons. Combining this protocol with double transfection to express a presynaptic marker and protein of interest allows us to locate presynaptic sites, their synaptic vesicle recycling capacity, and determine the role of the protein of interest.
