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JoVE Journal Genetics
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii
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Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

Article DOI: 10.3791/58044-v • 07:20 min • August 31st, 2018
August 31st, 2018 •
1, 1, 1
1Biological Sciences Department, Vanderbilt University

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Summary
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The aphid Aphis nerii colonizes on highly-defended plants in the dogbane family (Apocyanaceae) and provides numerous opportunities to study plant-insect interactions. Here, we present a series of protocols for the maintenance of plant and aphid cultures, and the generation and analysis of molecular and -omic data for A. nerii.

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Aphid Culturing Genetic Analysis Plant-insect Interactions Gene Expression Aphis Nerii Non-model System Evolution Ecology Molecular Mechanisms Low Cost Seedling Tray Germination Mix Soil Greenhouse Watering Moderate Soil Moisture Level Four Inch Round Pots Potting Soil Seedlings
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