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Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
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Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

Article DOI: 10.3791/58626-v • 10:19 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 3, 1, 3
1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, 2Department of Biomedical Engineering, Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science, Washington University School of Medicine

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Summary
Automatic Translation

This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.

Tags

Energy Metabolism Retinal Tissue Extracellular Flux Analysis Glycolysis Oxidated Phosphorylation Real-time Interventions Calibration Solution Sensor Cartridge Mitochondrial Stress Protocol Glycolysis Protocol Injector Port Volume Changes Assay Tissue Movement Artifact Base Medium Fresh Mouse Retinal Tissue
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